945 Galectin-12 Regulates the Immune Response in Skin Through Sebaceous Glands

Sebaceous glands are holocrine that helps maintain the skin barrier function by producing sebum, but also known to exert immunomodulatory effects. Through immunohistochemistry, flow cytometry, and immunoblotting, we found sebocytes expressed interleukin (IL)-4 receptor responded IL-4 produce high levels of CCL26, among other inflammatory mediators. Galectin-12 is a lipogenic factor in adipocytes resides intracellularly. Using galectin-12-knockdown (KD) epidermis-specific galectin-12 conditional knockout (cKO) mice, showed critical for IL-4-induced CCL26 expression. Moreover, using qPCR immunoblot analyses, induced PPARγ expression induction was dose-dependently ameliorated antagonist. Here, lower KD KO sebaceous glands. By gene ontology (GO) analysis, set ER stress-response genes significantly upregulated sebocytes. Treatment with pharmaceutical inducers stress suppressed IL-4, suggesting activation impairs signaling Thus, may promote response through suppressing stress. Finally, intradermal injection increased gland size leukocyte infiltrations, these responses were cKO mice. Similarly, mouse exhibited less prominent atopic dermatitis-like phenotype epicutaneous sensitization ovalbumin. identified regulatory role its effects on We propose promotes development Th2-mediated diseases, including dermatitis, mechanisms.